基于超高效液相色谱-四极杆/静电场轨道阱高分辨质谱技术的脑心通胶囊化学成分研究

孙志, 周霖, 李卓伦, 师莹莹, 刘丽伟, 周珮珮, 张俊, 王肖辉, 王松, 杨杰, 牛超, 侯朋艺, 卜鑫淼, 张晓坚

中国药学杂志 ›› 2018, Vol. 53 ›› Issue (19) : 1687-1695.

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中国药学杂志 ›› 2018, Vol. 53 ›› Issue (19) : 1687-1695. DOI: 10.11669/cpj.2018.19.014
论著

基于超高效液相色谱-四极杆/静电场轨道阱高分辨质谱技术的脑心通胶囊化学成分研究

  • 孙志1a,b, 周霖1a,b, 李卓伦1a,b, 师莹莹1a,b, 刘丽伟1a,b, 周珮珮1a,b, 张俊1a,b, 王肖辉1c, 王松1a,b, 杨杰1a,b, 牛超2, 侯朋艺3, 卜鑫淼2, 张晓坚1a,b*
作者信息 +

The Chemical Constituents Study of Naoxintong Capsules Based on UHPLC-Q-Orbitrap HRMS

  • SUN Zhi1a,b, ZHOU Lin1a,b, LI Zhuo-lun1a,b, SHI Ying-ying1a,b, LIU Li-wei1a,b, ZHOU Pei-pei1a,b, ZHANG Jun1a,b, WANG Xiao-hui1c, WANG Song1a,b, YANG Jie1a,b, NIU Chao2, HOU Peng-yi3, BU Xin-miao2, ZHANG Xiao-jian1a,b*
Author information +
文章历史 +

摘要

目的 为系统研究中药复方制剂脑心通胶囊的化学成分组成,本实验采用先进的超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UHPLC-Q-Orbitrap HRMS)技术建立一种快速准确识别脑心通胶囊中多种化学成分的定性分析方法。方法 本实验通过分析UHPLC-Q-Orbitrap HRMS技术捕捉未知化合物的一级精确相对分子质量和多级碎片信息,同时与对照品的保留时间和质谱数据信息进行比对,并结合相关参考文献以及Mass Bank、Chemical Book等网络数据库信息从而实现对药物中化学成分的准确快速定性。结果 本实验从脑心通胶囊中共鉴定出44种化学成分,其中主要包括黄酮类、醌类、萜类、及有机酸类等化学成分。结论 本实验建立的方法可快速、准确,系统地识别脑心通胶囊中多种化学成分,并为其质量控制、药效物质基础研究及进一步的临床应用提供坚实的理论依据和科学的研究思路。

Abstract

OBJECTIVE To establish a novel ultra-high performance liquid chromatography-quadrupole/orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) method to study the chemical composition of Naoxintong capsules rapidly and accurately. METHODS The information of accurate mass in full-MS and multistage fragment ions was obtained by the novel UHPLC-Q-Orbitrap technology. Chemical constituents were identified by comparing the relative retention time and the mass data of the reference substances, meanwhile consulting the reference literature or the Mass Bank, Chemical Book network database. RESULTS Forty-four compounds were finally identified in this study, mainly including flavones, authraquinones, terpenoids and organic acids. CONCLUSION A method is established in this study to identify various chemical constituents of Naoxintong capsules rapidly, accurately and systematically. What's more, our research will lay a sound theoretical foundation and propose a scientific study idea for the quality control improvement, bioactive components recognition and further clinical application of this herbal formulation.

关键词

脑心通胶囊 / 超高效液相色谱-四极杆/静电场轨道阱高分辨质谱 / 化学成分研究

Key words

Naoxintong capsules / UPLC-Q-Orbitrap HRMS / chemical constituents study

引用本文

导出引用
孙志, 周霖, 李卓伦, 师莹莹, 刘丽伟, 周珮珮, 张俊, 王肖辉, 王松, 杨杰, 牛超, 侯朋艺, 卜鑫淼, 张晓坚. 基于超高效液相色谱-四极杆/静电场轨道阱高分辨质谱技术的脑心通胶囊化学成分研究[J]. 中国药学杂志, 2018, 53(19): 1687-1695 https://doi.org/10.11669/cpj.2018.19.014
SUN Zhi, ZHOU Lin, LI Zhuo-lun, SHI Ying-ying, LIU Li-wei, ZHOU Pei-pei, ZHANG Jun, WANG Xiao-hui, WANG Song, YANG Jie, NIU Chao, HOU Peng-yi, BU Xin-miao, ZHANG Xiao-jian. The Chemical Constituents Study of Naoxintong Capsules Based on UHPLC-Q-Orbitrap HRMS[J]. Chinese Pharmaceutical Journal, 2018, 53(19): 1687-1695 https://doi.org/10.11669/cpj.2018.19.014
中图分类号: R917   

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基金

河南省高等学校重点科研项目基础研究计划资助(19A320070);常州四药临床药学科研基金资助(CZSYJJ6015);河南省科技计划项目资助(182102310243)
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